Nutrition
Volume 26, Issue 7 , Pages 842-848 , July 2010

High-fructose diet elevates myocardial superoxide generation in mice in the absence of cardiac hypertrophy

  • Kimberley Mellor, B.Sc.

      Affiliations

    • Department of Physiology, University of Melbourne, Melbourne, Victoria, Australia
    • ,
  • Rebecca H. Ritchie, Ph.D.

      Affiliations

    • Heart Failure Pharmacology, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia
    • ,
  • Greta Meredith, B.Sc.

      Affiliations

    • Department of Physiology, University of Melbourne, Melbourne, Victoria, Australia
    • ,
  • Owen L. Woodman, Ph.D.

      Affiliations

    • School of Medical Sciences, Royal Melbourne Institute of Technology, Melbourne, Victoria, Australia
    • ,
  • Margaret J. Morris, Ph.D.

      Affiliations

    • Department of Pharmacology, University of New South Wales, Sydney, New South Wales, Australia
    • ,
  • Lea M.D. Delbridge, Ph.D.

      Affiliations

    • Department of Physiology, University of Melbourne, Melbourne, Victoria, Australia
    • Corresponding Author InformationCorresponding author. Tel.: +61-3-8344-5853; fax: +61-3-8344-5897.

Received 20 October 2008 ,Accepted 10 August 2009.

  • Image Result

    Mean systolic blood pressure in the final week of dietary intervention (n=11–12/group). The horizontal bars depict an age effect. Data are presented as mean±SEM. # Age factor effect, P<0.05, two-wa

    Mean systolic blood pressure in the final week of dietary intervention (n=11–12/group). The horizontal bars depict an age effect. Data are presented as mean±SEM. # Age factor effect, P<0.05, two-way analysis of variance. cont, control; fruc, fructose.

  • Image Result
    (A) Cardiac weight index. Heart weight (milligrams) to body weight (grams) ratio at completion of a 6-wk dietary treatment (n=17–18/group). The horizontal bars depict an age factor effect. (B) Gene ex

    (A) Cardiac weight index. Heart weight (milligrams) to body weight (grams) ratio at completion of a 6-wk dietary treatment (n=17–18/group). The horizontal bars depict an age factor effect. (B) Gene expression level of β-MHC, a prohypertrophic gene in fructose-fed versus control-fed mice at 10 and 20 wk of age (normalized to 18S and determined relative to 10-wk-old control group, n=11–12/group). Data are presented as mean±SEM. # Age factor effect, P<0.05, two-way analysis of variance. β-MHC, β-myosin heavy chain; cont, control; fruc, fructose.

  • Image Result
    Nicotinamide adenosine dinucleotide phosphate–stimulated superoxide (O2−) production (counts per second per milligram of dry tissue) in fructose-fed and control-fed mice at completion of the 6-wk diet

    Nicotinamide adenosine dinucleotide phosphate–stimulated superoxide (O2−) production (counts per second per milligram of dry tissue) in fructose-fed and control-fed mice at completion of the 6-wk dietary intervention. Superoxide production in (A) ventricular tissue (n=6 tissue fragments/animal, n=15–17/group) and (B) thoracic aorta (n=3 aortic rings/animal, n=16–17/group). Data are presented as mean±SEM. ∗ Diet factor effect, P<0.05, two-way analysis of variance. cont, control; fruc, fructose.

  • Image Result
    Nox2 gene expression normalized to 18S and determined relative to the 10-wk-old control group. Fructose-fed versus control-fed mice at 10 and 20 wk of age (n=9–10/group). Data are presented as mean±SE

    Nox2 gene expression normalized to 18S and determined relative to the 10-wk-old control group. Fructose-fed versus control-fed mice at 10 and 20 wk of age (n=9–10/group). Data are presented as mean±SEM. cont, control; fruc, fructose.

  • Image Result
    Thx2 gene expression normalized to β-tubulin and determined relative to the 10-wk-old control group. Fructose-fed versus control-fed mice at 10 and 20 wk of age (n=5–6/group). Data are presented as me

    Thx2 gene expression normalized to β-tubulin and determined relative to the 10-wk-old control group. Fructose-fed versus control-fed mice at 10 and 20 wk of age (n=5–6/group). Data are presented as mean±SEM. cont, control; fruc, fructose; Thx2, thioredoxin-2.

  • Image Result
    Lipid peroxidation (ventricular TBARS) after 6-wk dietary treatment (n=13–15/group). Data are presented as mean±SEM. cont, control; fruc, fructose; TBARS, thiobarbituric acid-reactive substances.

    Lipid peroxidation (ventricular TBARS) after 6-wk dietary treatment (n=13–15/group). Data are presented as mean±SEM. cont, control; fruc, fructose; TBARS, thiobarbituric acid-reactive substances.

 This study was supported by Diabetes Australia, the National Heart Foundation, and the National Health and Medical Research Council, Australia.

PII: S0899-9007(09)00342-6

doi: 10.1016/j.nut.2009.08.017

Nutrition
Volume 26, Issue 7 , Pages 842-848 , July 2010

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