Nutrition
Volume 26, Issue 7 , Pages 835-841 , July 2010

Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats

  • Jean Guy LeBlanc, Ph.D.

      Affiliations

    • Centro de Referencia para Lactobacillos (CERELA-CONICET), Tucumán, Argentina
    • ,
  • Wilbert Sybesma, Ph.D.

      Affiliations

    • Wageningen Centre for Food Sciences, Wageningen, The Netherlands
    • NIZO Food Research, Ede, The Netherlands
    • ,
  • Marjo Starrenburg, Ph.D.

      Affiliations

    • Wageningen Centre for Food Sciences, Wageningen, The Netherlands
    • NIZO Food Research, Ede, The Netherlands
    • ,
  • Fernando Sesma, Ph.D.

      Affiliations

    • Centro de Referencia para Lactobacillos (CERELA-CONICET), Tucumán, Argentina
    • ,
  • Willem M. de Vos, Ph.D.

      Affiliations

    • Wageningen Centre for Food Sciences, Wageningen, The Netherlands
    • ,
  • Graciela Savoy de Giori, Ph.D.

      Affiliations

    • Centro de Referencia para Lactobacillos (CERELA-CONICET), Tucumán, Argentina
    • Universidad Nacional de Tucumán, Tucumán, Argentina
    • Corresponding Author InformationCorresponding author. Tel.: +54-381-431-0465; fax: +54-381-400-5600.
    • ,
  • Jeroen Hugenholtz, Ph.D.

      Affiliations

    • Wageningen Centre for Food Sciences, Wageningen, The Netherlands
    • NIZO Food Research, Ede, The Netherlands

Received 18 May 2009 ,Accepted 26 June 2009.

References 

  1. MRC Vitamin Study Research Group. Prevention of neural tube defects: results of the Medical Research Council Vitamin Study. Lancet. 1991;338:131–137
  2. Boushey CJ, Beresford SA, Omenn GS, Motulsky AG. A quantitative assessment of plasma homocysteine as a risk factor for vascular disease. Probable benefits of increasing folic acid intakes. JAMA. 1995;274:1049–1057
  3. Czeizel AE, Dudas I. Prevention of the first occurrence of neural-tube defects by periconceptional vitamin supplementation. N Engl J Med. 1992;327:1832–1835
  4. Danesh J, Lewington S. Plasma homocysteine and coronary heart disease: systematic review of published epidemiological studies. J Cardiovasc Risk. 1998;5:229–232
  5. Herbert V. Biochemical and hematologic lesions in folic acid deficiency. Am J Clin Nutr. 1967;20:562–572
  6. Hines JD, Halsted CH, Griggs RC, Harris JW. Megaloblastic anemia secondary to folate deficiency associated with hypothyroidism. Ann Intern Med. 1968;68:792–805
  7. Ma J, Stampfer MJ, Giovannucci E, Artigas C, Hunter DJ, Fuchs C, et al. Methylenetetrahydrofolate reductase polymorphism, dietary interactions, and risk of colorectal cancer. Cancer Res. 1997;57:1098–1102
  8. Konings EJ, Roomans HH, Dorant E, Goldbohm RA, Saris WH, van den Brandt PA. Folate intake of the Dutch population according to newly established liquid chromatography data for foods. Am J Clin Nutr. 2001;73:765–776
  9. O'Brien MM, Kiely M, Harrington KE, Robson PJ, Strain JJ, Flynn A. The North/South Ireland Food Consumption Survey: vitamin intakes in 18–64-year-old adults. Public Health Nutr. 2001;4:1069–1079
  10. Morris MC, Tangney CC. Is dietary intake of folate too low?. Lancet. 2007;369:166–167
  11. Scott JM. Folate and vitamin B12. Proc Nutr Soc. 1999;58:441–448
  12. Haberg SE, London SJ, Stigum H, Nafstad P, Nystad W. Folic acid supplements in pregnancy and early childhood respiratory health. Arch Dis Child. 2009;94:180–184
  13. Sauberlich HE, Kretsch MJ, Skala JH, Johnson HL, Taylor PC. Folate requirement and metabolism in nonpregnant women. Am J Clin Nutr. 1987;46:1016–1028
  14. McNulty H, Pentieva K. Folate bioavailability. Proc Nutr Soc. 2004;63:529–536
  15. Wang TT, Reisenauer AM, Halsted CH. Comparison of folate conjugase activities in human, pig, rat and monkey intestine. J Nutr. 1985;115:814–819
  16. Gregory JF. The bioavailability of folate. In:  Bailey LB editors. Folates in health and disease. New York: Marcel Dekker; 1995;p. 195–235
  17. Melse-Boonstra A, West CE, Katan MB, Kok FJ, Verhoef P. Bioavailability of heptaglutamyl relative to monoglutamyl folic acid in healthy adults. Am J Clin Nutr. 2004;79:424–429
  18. Lin MY, Young CM. Folate levels in cultures of lactic acid bacteria. Int Dairy J. 2000;10:409–413
  19. Sybesma W, Starrenburg M, Tijsseling L, Hoefnagel MH, Hugenholtz J. Effects of cultivation conditions on folate production by lactic acid bacteria. Appl Environ Microbiol. 2003;69:4542–4548
  20. Sybesma W, Starrenburg M, Kleerebezem M, Mierau I, de Vos WM, Hugenholtz J. Increased production of folate by metabolic engineering of Lactococcus lactis. Appl Environ Microbiol. 2003;69:3069–3076
  21. Wegkamp A, van Oorschot W, de Vos WM, Smid EJ. Characterization of the role of para-aminobenzoic acid biosynthesis in folate production by Lactococcus lactis. Appl Environ Microbiol. 2007;73:2673–2681
  22. Wegkamp A, Starrenburg M, de Vos WM, Hugenholtz J, Sybesma W. Transformation of folate-consuming Lactobacillus gasseri into a folate producer. Appl Environ Microbiol. 2004;70:3146–3148
  23. Sybesma W, Van Den Born E, Starrenburg M, Mierau I, Kleerebezem M, De Vos WM, et al. Controlled modulation of folate polyglutamyl tail length by metabolic engineering of Lactococcus lactis. Appl Environ Microbiol. 2003;69:7101–7107
  24. Terzaghi BE, Sandine WE. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975;29:807–813
  25. Horne DW, Patterson D. Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates. Clin Chem. 1988;34:2357–2359
  26. Clifford AJ, Wilson DS, Bills ND. Repletion of folate-depleted rats with an amino acid–based diet supplemented with folic acid. J Nutr. 1989;119:1956–1961
  27. Herbert V, Bertino JR. Folic acid. In:  Gryorgy P editors. The vitamins. New York: Academic Press; 1967;p. 243–276
  28. Cuskelly GJ, McNulty H, Scott JM. Effect of increasing dietary folate on red-cell folate: implications for prevention of neural tube defects. Lancet. 1996;347:657–659
  29. Clifford AJ, Jones AD, Bills ND. Bioavailability of folates in selected foods incorporated into amino acid–based diets fed to rats. J Nutr. 1990;120:1640–1647
  30. Walzem RL, Clifford AJ. Folate deficiency in rats fed diets containing free amino acids or intact proteins. J Nutr. 1988;118:1089–1096
  31. Brouwer IA, van Dusseldorp M, West CE, Meyboom S, Thomas CM, Duran M, et al. Dietary folate from vegetables and citrus fruit decreases plasma homocysteine concentrations in humans in a dietary controlled trial. J Nutr. 1999;129:1135–1139
  32. Klijn N, Weerkamp AH, de Vos WM. Genetic marking of Lactococcus lactis shows its survival in the human gastrointestinal tract. Appl Environ Microbiol. 1995;61:2771–2774
  33. Drouault S, Corthier G, Ehrlich SD, Renault P. Survival, physiology, and lysis of Lactococcus lactis in the digestive tract. Appl Environ Microbiol. 1999;65:4881–4886
  34. LeBlanc JG, Burgess C, Sesma F, de Giori GS, van Sinderen D. Lactococcus lactis is capable of improving the riboflavin status in deficient rats. Br J Nutr. 2005;94:262–267
  35. LeBlanc JG, Burgess C, Sesma F, Savoy de Giori G, van Sinderen D. Ingestion of milk fermented by genetically modified Lactococcus lactis improves the riboflavin status of deficient rats. J Dairy Sci. 2005;88:3435–3442
  36. LeBlanc JG, Rutten G, Bruinenberg P, Sesma F, de Giori GS, Smid EJ. A novel dairy product fermented with Propionibacterium freudenreichii improves the riboflavin status of deficient rats. Nutrition. 2006;22:645–651
  37. Crittenden RG, Martinez NR, Playne MJ. Synthesis and utilisation of folate by yoghurt starter cultures and probiotic bacteria. Int J Food Microbiol. 2003;80:217–222
  38. Holasova M, Fiedlerova V, Roubal P, Pechacova M. Biosynthesis of folates by lactic acid bacteria and propionibacteria in fermented milk. Czech J Food Sci. 2004;22:175–181
  39. Pompei A, Cordisco L, Amaretti A, Zanoni S, Matteuzzi D, Rossi M. Folate production by bifidobacteria as a potential probiotic property. Appl Environ Microbiol. 2007;73:179–185
  40. Hugenholtz J, Hunik J, Santos H, Smid E. Nutraceutical production by propionibacteria. Lait. 2002;82:103–112
  41. Pompei A, Cordisco L, Amaretti A, Zanoni S, Raimondi S, Matteuzzi D, et al. Administration of folate-producing bifidobacteria enhances folate status in Wistar rats. J Nutr. 2007;137:2742–2746

 This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) and the European Commission through contract QLK1-CT-2000-01376 (Nutracells).

PII: S0899-9007(09)00298-6

doi: 10.1016/j.nut.2009.06.023

Nutrition
Volume 26, Issue 7 , Pages 835-841 , July 2010

Article Tools

* Image Usage & Resolution