Dietary nucleotides and human immune cells. II. Modulation of PBMC growth and cytokine secretion

DNA-Na⁺ from fish soft roe (50 μg/mL) and RNA from bakers yeast (50 μg/mL or 1 mg/mL) significantly increases Inf-induced immune cell growth. Inf (0.33 μg/mL) induced significant proliferation of peripheral blood mononuclear cells compared with control casein (P = 0.0367), Triplicate measurements of peripheral blood mononuclear cells from 10 healthy subjects were investigated. Results are presented as mean dpm ± standard error of the mean. Differences are significant at P < 0.05. dpm, determinations per minute; Inf, influenza virus antigen.

Nucleotides dAMP, dCMP (50 μg/mL, 1 mg/mL), and dUMP (50 μg/mL) significantly increase Inf-induced immune cell growth. Triplicate measurements of peripheral blood mononuclear cells from 10 healthy subjects were investigated. Growth responses are presented as mean dpm ± standard error of the mean. Differences are significant at P < 0.05. dAMP, deoxyadenosine monophosphate; dCMP, deoxycytidine monophosphate; dpm, determinations per minute; dUMP, deoxyuridine monophosphate; Inf, influenza virus antigen.

Nucleotides dGMP and TMP, when cocultured with Inf, inhibit virus-induced growth of peripheral blood mononuclear cells. Responses are presented as dpm mean ± standard error of the mean (n = 10, P < 0.05). dGMP, deoxyguanosine monophosphate; dpm, determinations per minute; Inf, influenza virus antigen; TMP, thymidine monophosphate.