Oral administration of bifidobacterium longum culture condensate in a diet-restricted murine peritonitis model enhances polymorphonuclear neutrophil recruitment into the local inflammatory site

Hidemura, Akio; Saito, Hideaki; Fukatsu, Kazuhiko; Matsuda, Takeaki; Kitayama, Joji; Ikeda, Shigeo; Kang, Woodae; Nagawa, Hirokazu

doi:10.1016/S0899-9007(02)01060-2

« Previous Next »Nutrition
Volume 19, Issue 3 , Pages 270-274, March 2003

Oral administration of bifidobacterium longum culture condensate in a diet-restricted murine peritonitis model enhances polymorphonuclear neutrophil recruitment into the local inflammatory site

Akio Hidemura, MD
- Correspondence to: Akio Hidemura, MD, Department of Surgery, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
- Department of Surgery, Faculty of Medicine, Tokyo, Japan
Hideaki Saito, MD
- Surgical Center, The University of Tokyo, Tokyo, Japan
Kazuhiko Fukatsu, MD
- Department of Surgery, Faculty of Medicine, Tokyo, Japan
Takeaki Matsuda, MD
- Department of Surgery, Faculty of Medicine, Tokyo, Japan
Joji Kitayama, MD
- Department of Surgery, Faculty of Medicine, Tokyo, Japan
Shigeo Ikeda, MD
- Department of Surgery, Faculty of Medicine, Tokyo, Japan
Woodae Kang, MD
- Surgical Center, The University of Tokyo, Tokyo, Japan
Hirokazu Nagawa, MD
- Department of Surgery, Faculty of Medicine, Tokyo, Japan

Abstract

Objective

Dietary restriction impairs polymorphonuclear neutrophil (PMN) recruitment into the local inflammatory site, resulting in susceptibility to infection. Probiotics enhance host immunity via conditioning host intestinal microflora. Oral administration of Bifidobacterium longum culture condensate (BCC) in a diet-restricted murine peritonitis model may enhance PMN recruitment into the inflammatory site.

Methods

Male ICR mice (n = 40) were assigned in equal numbers to control or BCC groups and subjected to 75% restricted food intake for 7 d. During dietary restriction, controls received only standard mouse chow, whereas the BCC group received standard mouse chow containing 1% BCC. Mice were killed before (0 h) or after (2 or 4 h) intraperitoneal glycogen injection. Peritoneal lavage fluid and exudative cells were recovered by peritoneal lavage. Peritoneal exudative cell number was counted. Tumor necrosis factor-α, interleukin-6, macrophage inflammatory protein-2, and interleukin-10 concentrations in peritoneal lavage fluid were determined by enzyme-linked immunosorbent assay. CD11b, CD18, CD31, and CD62L expressions on circulating PMNs were measured by flow cytometry.

Results

Oral BCC administration upregulated PMN recruitment into the peritoneal cavity and increased peritoneal fluid cytokine concentrations as well as CD18 and CD62L expressions on circulating PMNs during glycogen-induced peritonitis.

Conclusions

Oral BCC administration in a diet-restricted murine peritonitis model augmented PMN recruitment into the inflammatory site by upregulating cytokine concentrations in the local inflammatory site and adhesion molecule expression on circulating PMNs. Oral BCC administration may be a favorable modality for improving dietary restriction-induced host immunosuppression.

Keywords: polymorphonuclear neutrophil, probiotics, Bifidobacterium longum, cytokine, adhesion molecule, recruitment