Nutrition
Volume 19, Issue 1 , Pages 41-46, January 2003

Dietary ribonucleotides increase antigen-specific type 1 T-helper cells in the regional draining lymph nodes in young BALB/cJ mice☆

Part of this study was presented in the Annual Meeting of Experimental Biology, 2001, held in Orlando, FL, March 31 to April 4, 2001.

  • Harumi Jyonouchi, MD

      Affiliations

    • Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA
    • Corresponding Author InformationCorrespondence to: Harumi Jyonouchi, MD, Division of Allergy/Immunology/ID/Pulmonology, Department of Pediatrics, UMDNJ (University of Medicine and Dentistry of New Jersey) 185 South Orange Ave., Newark, NJ 07103-2714, USA.
    • ,
  • Sining Sun, DDS

      Affiliations

    • Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • Timothy Winship, PhD

      Affiliations

    • Ross Products Division, Abbott Laboratories, Columbus, Ohio, USA
    • ,
  • Matthew J Kuchan, PhD

      Affiliations

    • Ross Products Division, Abbott Laboratories, Columbus, Ohio, USA

Abstract 

Objectives

We assessed the mechanisms of ribonucleotide action on type 1 T-helper cell (Th1) responses against ovalbumin (OVA) in Th2-biased BALB/cJ mice.

Methods

Mice were fed a ribonucleotide-free or ribonucleotide-supplemented diet and given OVA subcutaneously with incomplete Freund’s adjuvant at 3 and 6 wk. Costimulatory molecule expression (CD86 and CD154), the state of naive versus effecter/memory Th cells, and the frequency of OVA-specific resting versus activated Th1/Th2 cells were accessed in cells from the regional draining lymph nodes. OVA challenge increased CD86, but not CD154, expression. Effector/memory stage Th/cytotoxic T cells increased after the first and second OVA challenges.

Results

Dietary ribonucleotides did not affect the expression of any of these cell surface molecules. Antigen-specific Th1 and Th2 cells increased 10 d after the first OVA dose and 5 d after the second OVA dose. Further, dietary ribonucleotides increased OVA-specific resting and activated Th1 cells 10 d after the first OVA dose and decreased OVA-specific resting Th2 cells 5 d after the second OVA dose.

Conclusions

Dietary ribonucleotides may attenuate skewed Th2 responses by augmenting clonal expansion of OVA-specific Th1 cells, suppressing expansion of OVA-specific Th2 cells in Th2-biased BLAB/cJ mice, and not affecting antigen non-specific cell surface markers.

Keywords:  dietary ribonucleotides, type 1 and type 2 T-helper cells, antigen-specific T-helper cells, costimulatory marker expression

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☆ This study was supported in part by a grant from the Ross Product Division, Abbott Laboratories, Columbus, Ohio, USA.

PII: S0899-9007(02)00931-0

Nutrition
Volume 19, Issue 1 , Pages 41-46, January 2003

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